Health Advise 4u : Stay healthy and fit

    Subscribe to Updates

    Get the latest creative news from FooBar about art, design and business.

    What's Hot

    Should I Get Laser Hair Removal In The Winter?

    January 12, 2023

    What Is the Most Convenient Way to Refill Prescriptions?

    November 21, 2022

    6 Early symptoms of diabetes

    November 19, 2022
    Facebook Twitter Instagram
    • Demos
    • Buy Now
    Facebook Twitter Instagram
    Health Advise 4u : Stay healthy and fit Health Advise 4u : Stay healthy and fit
    Subscribe
    • Home
    • Dieting
    • Doctor
    • Fitness
    • Health
    • Pregnancy
    • Treatment
    • Weight Loss
    Health Advise 4u : Stay healthy and fit
    Home»Health»How has the western blotting technique improved?
    Health

    How has the western blotting technique improved?

    Ryan LucaBy Ryan LucaFebruary 9, 2022No Comments3 Mins Read
    Facebook Twitter Pinterest LinkedIn Tumblr Email
    Share
    Facebook Twitter LinkedIn Pinterest Email

    ​​Western blotting revolutionized the ability to measure proteins in samples. While the original development of this technique was an advanced tool to electrophoretically separate proteins when George Stark, Harry Towbin, and W. Neal Burnette independently developed what we refer to as western blotting. In fact, it is still advanced, but through the gifts that new technology brings, there have been improvements to the process that keeps the western blot method a cutting edge tool. Keep reading as we go through how the western blot technique has improved thanks to advancements in technology.

    Bio-Rad’s Stain-free Technology

    Thanks to the complex nature and multiple steps within the western blot method and the fact that along the way to a result are several steps that are manually made and not automated, the process can cause errors. To test the results and when errors are present to identify where in the process they occurred required staining and de-staining of gels. Bio-Rad has advanced this process with stain-free technology. The process is automated, and during the process, it confirms accurate and correct methods at each step of the western blotting process. The advancement means the western blot test is now more efficient and that Quality Control can occur at each level both within the gel and on the membrane.

    Increased Multiplexing Capabilities with Fluorescence

    The standard has been Chemiluminescent reagents, but with the adaptation of fluorescence reagents, the western blot method expands with a greater range that surpasses the options presented by chemiluminescent reagents. The difference is that when fluorescent reagents are put in use, the tester can address the presence of multiple proteins at the same time.

    What has changed in The Western Blot Method?

    One of the more significant changes is that new advancements mean faster outcomes and results with greater accuracy. Being able to test for correctness at each stage of the test is an incredible time-saver. Knowing that the results “thus far” are accurate speeds up the entire process. Scientists no longer have to wait until the end to see if the visuals are correct. They no longer have to backtrack one step at a time, adding stain or taking it away to make sure that step is correct. In addition, the Western blot method has become more efficient, which means higher accuracy at each stage of the test.

    The use of fluorescent reagents and the fact that you can isolate more than one protein at a time means you do fewer tests. For example, you no longer have to run one test for protein group A and another for protein group B. You can do that at the same time.

    Moving Forward with the Western Blot Method

    Both of these advancements are relatively new. As they become more widely used, you can bet that additional advancements will occur. One of the possibilities is an increased range of fluorescent reagents, which may make the western blotting method much faster and more accurate. A significant change is a decrease in time, which means testing becomes more efficient and the applications become more mainstream.

    Share. Facebook Twitter Pinterest LinkedIn Tumblr Email
    Ryan Luca
    • Website

    Related Posts

    Should I Get Laser Hair Removal In The Winter?

    January 12, 2023

    What Is the Most Convenient Way to Refill Prescriptions?

    November 21, 2022

    6 Early symptoms of diabetes

    November 19, 2022
    Add A Comment

    Comments are closed.

    Top Posts

    How to talk to your family doctor about sensitive problems

    July 9, 2021

    Bootcamp Fitness – a great way to fit in a hurry

    June 17, 2021

    All you need to know about holistic doctors

    June 16, 2021

    Cardio fitness machine – amazing facts to know

    May 15, 2021
    Recent Posts
    • Should I Get Laser Hair Removal In The Winter?
    • What Is the Most Convenient Way to Refill Prescriptions?
    • 6 Early symptoms of diabetes
    • What is Impedance Flow Cytometry and How Can It Benefit Your Research?
    • The Disability Aids to Make Life Easier
    Top Reviews
    Archives
    • January 2023
    • November 2022
    • October 2022
    • September 2022
    • July 2022
    • June 2022
    • May 2022
    • April 2022
    • March 2022
    • February 2022
    • November 2021
    • October 2021
    • July 2021
    • June 2021
    • May 2021
    • April 2021
    • March 2021
    • February 2021
    • January 2021
    • December 2020

    Facebook Twitter Instagram Pinterest Vimeo YouTube
    • Home
    • Get In Touch
    © 2021 Health Advise 4u- All Rights Reserved.

    Type above and press Enter to search. Press Esc to cancel.